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Abnormal lipid-linked oligosaccharides in class E Thy-1-negative mutant lymphomas.

I S Trowbridge·R Hyman
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Abstract

The glycosylation defect of Thy-1-mutant lymphomas of the class E complementation group has been identified as a block in the synthesis of the lipid-linked oligosaccharide precursor of the asparagine-linked oligosaccharides of glycoproteins. Two major lipid-linked oligosaccharides were isolated from the mutant cells. Both oligosaccharides were smaller than the lipid-linkid oligosaccharides of wild-type lymphomas and, in contrast to the lipid-linked oligosaccharides isolated from wild-type cells, both were resistant to digestion with endoglycosidase H. The oligosaccharides of newly synthesized polypeptides in class E Thy-1-cells were also resistant to endoglycosidase H digestion, providing strong evidence that they are derived from the abnormal lipid-linked oligosaccharides.

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AnimalsCell LineGlycoproteinsIsoantigensLipid MetabolismLymphomaMembrane ProteinsMiceMutationNeoplasm ProteinsNeoplasms, ExperimentalOligosaccharidesJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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BibTeXRIS

I S Trowbridge, R Hyman. 1979. Abnormal lipid-linked oligosaccharides in class E Thy-1-negative mutant lymphomas.. https://doi.org/10.1016/0092-8674(79)90258-7

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