PubMed · 4782504
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V R Zhovtyĭ, M V Borzov. 1973. [Dermatitis in workers processing mussels].. https://pubmed.ncbi.nlm.nih.gov/4782504/
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A solid-phase luminescent immunoassay (LIA), based on the light emission produced as a result of the oxidation of Pholas dactylus luciferin by horseradish peroxidase (HRP) in the presence of molecular oxygen, was developed for human chorionic gonadotropin (hCG). The light emitted in the presence of diethyldithiocarbamate permitted the detection of 0.1 fmol HRP. HRP retained most of its light-emitting capacity after coupling with purified anti-hCG antibody by glutaraldehyde. The LIA involved immobilization of the antigen in plastic tubes coated with purified anti-hCG antibody and detection of the immunocomplex by light emission in the presence of Pholas luciferin. Light emission was linear for antigen concentration within the range 0.5-100 ng/ml. LIA correlates reasonably well with RIA and has a comparable sensitivity (0.5 ng hCG/ml).
Phagocytosis of human erythrocytes (rbc) by hemocytes of the mussel Mytilus edulis was found to be influenced by four heterologous lectins. The effects were examined in the absence of Ca++ ions under three experimental conditions: when the lectins were bound to 1) both hemocytes and rbc, 2) only hemocytes, but not to rbc, and 3) only rbc, but not to hemocytes. The lectins used included: albumen gland agglutinin from Helix pomatia (HPA), wheat germ agglutinin (WGA), Ricinus-120 (Ric-120) and Concanavalin-A (Con A). HPA, WGA and Ric-120, for which both hemocytes and A-rbc possess receptors, strongly enhanced uptake of A-rbc. This lectin-mediated phagocytosis was abolished by addition of specific sugars either to lectin-pretreated rbc (HPA, WGA) or to a pretreated hemocyte monolayer (Ric-120); this indicated the stimulation of phagocytosis by the binding of lectin to carbohydrate determinants at the surface of hemocytes and target cells. On the other hand, HPA which binds to hemocytes, but not to O-rbc, did not influence phagocytosis of these rbc; and Con A which binds to A-rbc, but not to hemocytes, also failed to stimulate phagocytosis. These findings reveal the importance of carbohydrate determinants on the surface of hemocytes as well as on target cells in recognition and in lectin-mediated phagocytosis of foreign cells by Mytilus hemocytes.