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Normal immunosuppressive protein purification and quantitative estimation experiments.

D Nelken·R Goren·H Ovadia·N Hanna
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Abstract

An improved method for the preparation and purification of normal immunosuppressive protein (NIP) is described. The purified material has a molecular weight between 10,000 and 25,000. Its biological and serological activity is approximately 10--20 times higher than that of the crude fraction. An antibody to normal immunosuppressive protein prepared in rabbits made the quantitative estimation of NIP by a haemaggluination inhibition test possible. Similarly, a very sensitive assay for the quantitative determination of NIP by its inhibitory effect on the proliferation of EL-4 tumor cells is also described. Eluates prepared from polyacrylamide gels were active in inhibiting EL-4 tumor cell proliferation and neutralized the anti-NIP activity in the haemagglutination inhibition test.

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AnimalsBlood ProteinsElectrophoresis, Polyacrylamide GelHemagglutination Inhibition TestsHumansImmune SeraImmunosuppressive AgentsMolecular WeightNeoplasms, ExperimentalRabbitsJournal Article

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D Nelken, R Goren, H Ovadia, N Hanna. 1979. Normal immunosuppressive protein purification and quantitative estimation experiments.. https://doi.org/10.1016/0022-1759(79)90193-5

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