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PubMed · 48265

Correlation of the critical micelle concentrations of surfactants with their effects on a bacterial demethylase.

J T Pinto·W R Frisell
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Abstract

The activity of a sarcosine dehydrogenase isolated from a strain of Pseudomonas is enhanced by the addition of Triton X-100, Brij 35, and Tween 80, and is inhibited by deoxycholate and Sarkosyl NL-97. 2,6-Dichlorophenolindophenol, which is used as the oxidant in the dehydrogenase assay, has also been employed as an indicator in the spectrophotometric determination of the critical micelle concentrations (CMC) of both the nonionic and anionic detergents under conditions optimal for the enzyme analyses. A correlation between the activation or inhibitory activities of the surfactants and their CMC values has been established.

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2,6-DichloroindophenolDeoxycholic AcidDetergentsEnzyme ActivationMicellesOxidoreductases, N-DemethylatingPolyethylene GlycolsPolysorbatesPseudomonasSarcosineSpectrophotometryJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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J T Pinto, W R Frisell. 1975. Correlation of the critical micelle concentrations of surfactants with their effects on a bacterial demethylase.. https://doi.org/10.3181/00379727-148-38672

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PubMed1979

Inhibition of NADH-methemoglobin reductase by organic phosphates.

The organic phosphate allosteric effectors of hemoglobin, inositol hexaphosphate, 2,3-diphosphoglycerate, and ATP, interact with NADH-methemoglobin reductase (NADH-diaphorase). Significant inhibitory effects on the enzyme were found when dichlorophenolindophenol, or ferricyanide were used as electron acceptors in place of methemoglobin. In contrast, apparent stimulation of enzyme activity was observed when adult human methemoglobin was used as the electroganic phosphate on the rate of reaction due to its interaction with the substrate methemoglobin to produce the favored T type of quaternary conformation. The inhibitory effect of inositol hexaphosphate on the enzyme is associated with a perturbation in the reactivity of essential sulfhydryl group(s) on the enzyme. It is suggested that the interaction of the organic phosphate with the enzyme as well as with the substrate is significant in determining the overall rate of methemoglobin reduction.

2,6-Dichloroindophenol↗