PubMed Health⌕ Search

PubMed · 484992

A code for reporting and comparing results in phage typing.

Abstract

This code, like the others proposed, involves the definition of a characteristic reaction for each system. Each reaction must be positive or negative. As regards the interpretation of results, it is worth noting that if the bacteriophage typing system uses not less than 5 phages, there is no problem for interpreting the results of small series of 10 to 20 strains, whatever the code used. But should the system use more than 5 phages, the advantage offered by the octal code becomes obvious because, without computer, the manual coding for comparing the results of various series is always possible. Beyond 6 phages, the binary pattern reporting, unintelligible without correction, is possible with an octal number not exceeding 7 digits for a pattern corresponding to a 21-phage set. When very large series of strains are considered, the coding can be completely done and printed out by any computer through a very simple program. This proposed code is not only rational but also a useful method even if the number of phages used is larger than 20 or 30, but after a computer becomes necessary however the coding system used.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

A Audurier, F Chalons, M Toucas. 1979. A code for reporting and comparing results in phage typing.. https://pubmed.ncbi.nlm.nih.gov/484992/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

Molecular markers with potential to replace phage typing for Salmonella enterica serovar typhimurium.

Using Amplified Fragment Length Polymorphism (AFLP) analysis of isolates from 23 phage types, we isolated 11 molecular markers that are potentially useful for molecular typing of Salmonella enterica serovar typhimurium. We tested these and 11 previously studied markers for their ability to discriminate among isolates and for correlation of their distribution with phage types. The Simpson's index of discriminatory power for the molecular markers is 0.96. One hundred and twenty one isolates from 33 phage types tested were divided into 51 types which are further grouped into 24 patterns. Eight patterns can unambiguously identify 8 phage types and a further 12 correlated with phage type distribution, showing the usefulness of these markers for molecular phage typing.

Bacteriophage Typing↗

Complete nucleotide sequences of 84.5- and 3.2-kb plasmids in the multi-antibiotic resistant Salmonella enterica serovar Typhimurium U302 strain G8430.

The multi-antibiotic resistant (MR) Salmonella enterica serovar Typhimurium phage type U302 strain G8430 exhibits the penta-resistant ACSSuT-phenotype (ampicillin, chloramphenicol, streptomycin, sulfonamides and tetracycline), and is also resistant to carbenicillin, erythromycin, kanamycin, and gentamicin. Two plasmids, 3.2- and 84.5-kb in size, carrying antibiotic resistance genes were isolated from this strain, and the nucleotide sequences were determined and analyzed. The 3.2-kb plasmid, pU302S, belongs to the ColE1 family and carries the aph(3')-I gene (Kan(R)). The 84.5-kb plasmid, pU302L, is an F-like plasmid and contains 14 complete IS elements and multiple resistance genes including aac3, aph(3')-I, sulII, tetA/R, strA/B, bla(TEM-1), mph, and the mer operon. Sequence analyses of pU302L revealed extensive homology to various plasmids or transposons, including F, R100, pHCM1, pO157, and pCTX-M3 plasmids and TnSF1 transposon, in regions involved in plasmid replication/maintenance functions and/or in antibiotic resistance gene clusters. Though similar to the conjugative plasmids F and R100 in the plasmid replication regions, pU302L does not contain oriT and the tra genes necessary for conjugal transfer. This mosaic pattern of sequence similarities suggests that pU302L acquired the resistance genes from a variety of enteric bacteria and underscores the importance of a further understanding of horizontal gene transfer among the enteric bacteria.

Bacteriophage Typing↗