Augmented uptake of neuraminidase-treated sheep red blood cells: participation of opsonic factors.

J R Schmidtke; R L Simmons

doi:10.1093/jnci/54.6.1379

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Augmented uptake of neuraminidase-treated sheep red blood cells: participation of opsonic factors.

J R Schmidtke·R L Simmons

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Abstract

Neuraminidase from Vibrio cholerae (VCN) was used to treat sheep red blood cells (SRBC) which were then incubated in vitro with murine peritoneal macrophages. The uptake of VCN-treated SRBC by macrophages was greater than the uptake of SRBC not treated with VCN. SRBC opsonized with normal mouse serum (NMS) were taken up to a greater extent than untreated SRBC. SRBC treated with VCN and opsonized with NMS were phagocytosed to a greater extent than untreated SRBC, VCN-treated SRBC, or opsonized SRBC. Evidence demonstrated that factors in serum from normal C3H/HeJ mice augmented the uptake of VCN-treated SRBC in greater amounts than of normal SRBC. These findings were discussed in relation to the increased immunogenicity of neuraminidase-treated cells.

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Animals Epitopes Erythrocytes Female Macrophages Mice Mice, Inbred C3H Neuraminidase Opsonin Proteins Phagocytosis Sheep Vibrio cholerae Journal Article Research Support, U.S. Gov't, P.H.S.

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J R Schmidtke, R L Simmons. 1975. Augmented uptake of neuraminidase-treated sheep red blood cells: participation of opsonic factors.. https://doi.org/10.1093/jnci%2F54.6.1379

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