Serum calcium ion activity. Some aspects on methodological differences and intraindividual variation.

L Larsson; S Ohman

doi:10.1016/s0009-9120(79)80141-1

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Serum calcium ion activity. Some aspects on methodological differences and intraindividual variation.

L Larsson·S Ohman

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Abstract

Sera were analyzed for calcium ion activity on the Orion 99-20 and SS-20 systems. Samples from 84 healthy subjects were analyzed on both systems and, because of a change of electrode composition, 74 of them were reanalyzed after this change. Additional 89 were analyzed on only SS-20. The distribution of serum calcium ion activity was close to Gaussian. The difference between the 99-20 and SS-20 readings was 0.048+/-0.017 mmol/liter (range -0.01 to 0.08) before the electrode change and -0.085+/-0.027 mmol/liter (range -0.14 to 0.02) after. The reference ranges were 1.02--1.23 mmol/liter for 99-20 and 0.93--1.20 mmol/liter for SS-20 before the electrode change. The following characteristics may contribute to differences in the readings: higher temperature, higher flow rate, new calcium and reference electrodes, new liquid junction construction and a new pumping procedure. For 74 subjects the determination of serum calcium ion activity (99-20 system) was performed with a five year interval and the difference between the first and second measurements was -0.0046+/-0.0168 mmol/liter (mean +/-SD), ranging from -0.04 to 0.03 mmol/liter. This corresponded to an intraindividual variation of 1.5%. The corresponding variations for serum total calcium and corrected calcium were 4.8% and 3.3% respectively.

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Adolescent Adult Aged Calcium Cations, Divalent Electrochemistry Electrodes Female Humans Male Mathematics Middle Aged Comparative Study Journal Article

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L Larsson, S Ohman. 1979. Serum calcium ion activity. Some aspects on methodological differences and intraindividual variation.. https://doi.org/10.1016/s0009-9120(79)80141-1

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