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A modified quantitative precipitin test which is rapid, sensitive and reproducible.

J P Raney·B D McLennan
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Abstract

A modification of the standard quantitative precipitin test is described. The new procedure, which utilizes polyethylene glycol (PEG) at both the 37 degrees C and 4 degrees C incubation stages, provides a convenient quantitative precipitin test assay which is more reliable and much faster than the standard assay. Moreover, the modified or PEG assay gives a quantitative measure of antibody concentration even when antisera of low titre are tested. The sensitivity of the capillary-tube test is also enhanced when the supernatant solutions contain PEG.

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AnimalsAntigen-Antibody ComplexBinding, CompetitiveCattleHaptensPolyethylene GlycolsPrecipitin TestsPrecipitinsRabbitsReference StandardsSolubilityTemperatureTime FactorsJournal Article

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J P Raney, B D McLennan. 1979. A modified quantitative precipitin test which is rapid, sensitive and reproducible.. https://doi.org/10.1016/0022-1759(79)90126-1

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