Mouse cell-mediated lympholysis assay in serum-free and mouse serum-supplemented media: culture conditions and genetic factors.

A B Peck; F H Bach

doi:10.1111/j.1365-3083.1975.tb02599.x

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Mouse cell-mediated lympholysis assay in serum-free and mouse serum-supplemented media: culture conditions and genetic factors.

A B Peck·F H Bach

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Abstract

We describe a miniaturized cell-mediated lympholysis assay for measuring the activity of cytotoxic lymphocytes generated in mouse mixed leukocyte cultures (MLC), using either serum-free or mouse serum-supplemented media. Mixed reactions of strain combinations possessing various genetic differences at H-2 produced cytotoxic lymphocytes with cytolytic activity against cells of the donor used in the sensitization phase. Results suggest that both serologically defined and lymphocyte-defined determinants can act as effective target antigens on target cells. In contrast, no cytotoxic lymphocytes were generated against products of non-H-2 loci--M-locus, theta, or other non-H-2 antigens--that induce MLC activation.

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Animals Cytotoxicity Tests, Immunologic Epitopes Lectins Lipopolysaccharides Lymphocyte Activation Lymphocytes Mice Mice, Inbred Strains Polysaccharides, Bacterial Time Factors Journal Article Research Support, U.S. Gov't, P.H.S.

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A B Peck, F H Bach. 1975. Mouse cell-mediated lympholysis assay in serum-free and mouse serum-supplemented media: culture conditions and genetic factors.. https://doi.org/10.1111/j.1365-3083.1975.tb02599.x

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