[Dependence of heterochromatin differential staining on the time of its reduplication and the degree of condensation].

L P Tupitsyna; A F Zakharov; T G Tsvetkova

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PubMed · 49115

[Dependence of heterochromatin differential staining on the time of its reduplication and the degree of condensation].

L P Tupitsyna·A F Zakharov·T G Tsvetkova

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Abstract

A comparison of the chromosomes banding pattern after G-and C-staining with the time of DNA reduplication and the degree of chromosome condensation, was carried out using Chinese hamster metaphase chromosomes. Chromosome condensation was studied under 5-bromodeoxyuridine and 5-bromodeoxycytidine treatment. All the chromosomal segments stained with C-technique are also stainable with G-technique, while only some G-positive segments are capable to be C-bands. C-bands are heterochromatic segments characterized by extremely late replication and great delay in condensation under the analog action, while G-bands are segments with earlier labelling and irregular decondensation. The data obtained suggest a close correlation between the capability of chromosomal region of G- and C- staining and the degree of its heterochromatinization.

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Aneuploidy Animals Autoradiography Bromodeoxycytidine Bromodeoxyuridine Cell Line Cells, Cultured Chromosomes Cricetinae Cytological Techniques DNA Replication Heterochromatin Karyotyping Mitosis Staining and Labeling Time Factors Tritium English Abstract Journal Article

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L P Tupitsyna, A F Zakharov, T G Tsvetkova. 1975. [Dependence of heterochromatin differential staining on the time of its reduplication and the degree of condensation].. https://pubmed.ncbi.nlm.nih.gov/49115/

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