PubMed · 4923507
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E Maldyk. [Electrolytic bone decalcification].. https://pubmed.ncbi.nlm.nih.gov/4923507/
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Demineralization of dentin specimens proceeds at a faster rate than that of enamel. Although this is generally accepted, a quantification of the rate of formation of root lesions is hampered by the shrinkage of the lesions when these are dried prior to microradiographic analysis. This leads to a significant underestimation of the lesion depth and total mineral loss. The aim of this paper was to quantitate the rate of mineral loss during root lesion formation in vitro and to determine the shrinkage of root specimens as a result of drying. Unerupted roots of human teeth were subjected to a demineralizing system of 0.1 mol/L lactate buffer (pH = 4.8) with 0.2 mmol/L methanehydroxydiphosphonate during four, 11, 22, and 44 days. The root lesions were assessed by quantitative microradiography. The demineralizing solutions were analyzed to determine the amounts of root tissue dissolved. A comparison of these two sets of data showed that, with the demineralizing system used, root lesions may shrink up to 62%. Fixation of the specimens in fixative did not affect this shrinkage. Chemical analysis showed that mineral loss proceeded linearly with time. From the data-sets of this study, a model was developed to compensate for the shrinkage in the dentin specimens. In this way, it was possible to calculate the lesion depth at four demineralization times as being 130, 220, 320, and 530 microns, respectively. These values were in agreement with a microscopic determination of the lesion depth.
The recent interest for highly sophisticated techniques of dental tissue preparation aiming to display very particular structures, moved the AA. to improve the literature suggestions. In particular they made TEM and SEM observations of transitional zones between healthy and normal pulp and dentin after decalcification and trypsin at different concentrations treatment. The images obtained draw in the attention the study facilities of a technique that really removes all the non collagenic material. The data obtained in the pericellular zones also allowed some interventions in the recent literature discussion about inter-odontoblastic fibres.
By using a combination of histologic methods (decalcified and nondecalcified sections) the morphologic information on topological characteristics of periodontal disease is broader. Inflammatory parameters of disease progression and stagnation are well documentable. Exclusively ground stained sections of nondecalcified specimen of the local relationship of gingiva, calculus, and plaque are able to characterize the topology of the periodontal disease progress and the typical liquid phase of subgingival plaque.