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Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity.

R V Lachica·C Genigeorgis·P D Hoeprich
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Abstract

Based on the metachromatic property of Toluidine Blue O, three, convenient agar-diffusion methods have been developed that enable detection of the nuclease of Staphylococcus aureus at concentrations as low as 0.005 mug/ml in agar and broth cultures. The interactions of agar and deoxyribonucleic acid with Toluidine Blue O are discussed.

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AgarBacteriological TechniquesColoring AgentsDNADeoxyribonucleasesHydrolysisMethodsMicrococcusStaphylococcusJournal Article

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BibTeXRIS

R V Lachica, C Genigeorgis, P D Hoeprich. 1971. Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity.. https://doi.org/10.1128/am.21.4.585-587.1971

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