PubMed · 4942295
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R Hoigné, U Klein. 1971. [Urinary bacteriology in practice].. https://pubmed.ncbi.nlm.nih.gov/4942295/
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Urine samples from 358 asymptomatic males were screened for urethral inflammation by the leukocyte esterase (LE) test and for Chlamydia trachomatis by the ligase chain reaction (LCR). LE and LCR positivity rates were 7.5% (27 of 358 samples) and 2.8% (10 of 358 samples), respectively. Eight of the 10 LCR-positive samples were detected by the LE screening test. The urine LE prescreening test in combination with the LCR assay may be a reasonable approach for genitourinary chlamydial disease control.
Recovery rates from Middlebrook 7H11-selective 7H11 biplates and Lowenstein-Jensen slants (LJ) used with BACTEC 12B vials were compared for 5,399 specimens. For 578 specimens that were inoculated onto three media, 580 mycobacteria were isolated, including 277 (48%) Mycobacterium avium complex isolates, 230 (40%) Mycobacterium tuberculosis isolates, and 73 (12%) other mycobacteria. For BACTEC 12B vials, 506 (87%) cultures were positive, 45 (8%) were negative, and 29 (5%) were lost to contamination; for 7H11-7H11-selective biplates, 469 (81%) cultures were positive, 95 (16%) were negative, and 16 (3%) were lost to contamination; for LJ, 230 (40%) cultures were positive, 111 (19%) were negative, and 239 (41%) were lost to contamination. For routine cultures, use of plate media is superior to use of LJ.