PubMed · 4943874
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1971. Hazard: dye dilution pump.. https://pubmed.ncbi.nlm.nih.gov/4943874/
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Results of previous investigations on the effects of simulated microgravity (thermoneutral (34.5 degrees C) head-out water immersion, WI) have indicated that plasma volume (PV) increases initially and thereafter decreases to attain values below the pre-immersion level. In these cases, changes in hematocrit (Hct) and hemoglobin concentration (Hgb) were used as indicators of relative changes in PV. In order to test whether changes in Hct and Hgb are accurate measures of changes in PV during simulated microgravity, direct measurements of PV were performed with a modified Evans blue dye dilution technique before, during, and after a 12 h WI experiment. Furthermore, PV was determined with the same technique before, during, and after acute 6 degrees head-down tilt (HDT). Changes in PV were then compared with changes calculated from changes in Hct and Hgb.
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In this report a number of practical methods for the measurement of skeletal muscle blood flow in humans are presented and discussed. Special attention has been paid to the problems concerning representativeness for muscle blood flow (muscle vs other tissues and small muscle segments vs skeletal musculature of the total body) as well as the demand for steady state.