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Isotopic method for estimating microbial cell volumes.

D F Reid·H A Frank
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Abstract

Reid, Della F. (University of Hawaii, Honolulu), and Hilmer A. Frank. Isotopic method for estimating microbial cell volumes. J. Bacteriol. 92:639-644. 1966.-A sensitive isotopic method was developed for estimating the mean cell volume in small numbers of bacterial spores by measuring directly the extracellular fluid volume when cells are present in a saturated inulin solution containing inulin-C(14) at low specific activity. The method minimizes errors due to solute adsorption to the cells and avoids errors arising from differences in cell packing. The effects of these sources of error on cell volume and intercellular space estimations made with nonpenetrating solutes are discussed.

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Bacillus subtilisBacteriological TechniquesCarbon IsotopesChromatography, PaperInulinRadiometryJournal Article

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D F Reid, H A Frank. 1966. Isotopic method for estimating microbial cell volumes.. https://doi.org/10.1128/jb.92.3.639-644.1966

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