Cross-linked informofers.

V V Prosvirnin; S Ruzidic; O P Samarina

doi:10.1093/nar/7.6.1649

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PubMed · 503864

Cross-linked informofers.

V V Prosvirnin·S Ruzidic·O P Samarina

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Abstract

The proteins of 30S RNP particles containing pre-mRNA (hnRNA) were cross-linked with bifunctional reagents (dimethyl-suberimidate and dimethyl-3,3'-dithiobispropionimidate). Further treatment with 1 or 2 M NaCl dissociates all RNA from protein. However, a significant part of protein particles--informofers being cross-linked survived high salt treatment. Their sedimentation coefficients were close to those of original particles. No RNA could be detected in the informofers even after labeling the cells with a precursor for a long period of time. Sodium dodecylsulfate or urea dissociated cross-linked informofers into oligomeric polypeptides. They could be dissociated by beta-mercaptoethanol treatment if a reversible cross-linked reagent had been used. The resulting polypeptides were represented by informatin. RNP particles (30S RNP or poly-particles) were reconstituted upon mixing of cross-linked informofers with pre-mRNA and removal of 2 M NaCl.

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Animals Carcinoma, Ehrlich Tumor Cross-Linking Reagents Dimethyl Suberimidate Disulfides Electrophoresis, Polyacrylamide Gel Imidoesters Liver Mice Molecular Weight Nucleoproteins RNA, Messenger Rats Ribonucleoproteins Journal Article

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V V Prosvirnin, S Ruzidic, O P Samarina. 1979-11-24. Cross-linked informofers.. https://doi.org/10.1093/nar%2F7.6.1649

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