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PubMed · 504790

Radioimmunoassay for phencyclidine.

L S Rosenberg·H Van Vunakis
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Abstract

A sensitive and specific radioimmunoassay has been developed for the drug phencyclidine (PCP). Levels as low as 50 pg (200 femto-moles) of PCP can be detected. The specificity of the antisera has been investigated by inhibiting the [3H]PCP-anti PCP reaction with the parent drug, metabolites, and other related compounds. Three urinary metabolites, 4-phenyl-4-piperidinocyclohexanol, 1-(1-phenylcyclohexyl)-4-hydroxypiperidine and 1-phenylcyclohexylamine inhibit 2,5, and 0.002% as effectively as PCP respectively. Plasma samples supplemented with PCP (1.2 to 10,000 ng/ml) can be analyzed directly by this procedure.

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AnimalsCross ReactionsMethodsPhencyclidineRabbitsRadioimmunoassayJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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L S Rosenberg, H Van Vunakis. 1979. Radioimmunoassay for phencyclidine.. https://pubmed.ncbi.nlm.nih.gov/504790/

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