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Complement proteins and macrophages. 1. Quantitative estimation of factor B produced by mouse peritoneal macrophages.

Y Kawamoto·M Ueda·H Ichikawa·A Miyama
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Abstract

Cobra venom factor was used for the detection of factor B synthesized by mouse peritoneal macrophages. This method was shown to be specific for factor B assay by neutralization by antimouse factor B antibody. The amount of factor B in the culture supernatant, assessed by this method, was found to be dependent on the medium used for cultivation of macrophages. The addition of 25% L cell-conditioned medium to minimal essential medium (LCM-MEM) enhanced the production of factor B and also of lysozyme. Kinetic analysis in LCM-MEM showed that factor B produced by 6 x 10(4) cells/cm2 increased up to 72 hr and reached a plateau at 96 hr. The amounts of factor B and lysozyme produced in LCM-MEM depended upon the number of macrophages. Production of factor B was completely inhibited by 1 microgram of cycloheximide per ml and was restored by its removal.

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AnimalsColchicineComplement Factor BComplement System ProteinsCulture MediaCycloheximideEnzyme PrecursorsFucoseHemolysisKineticsMacrophagesMaleMiceJournal Article

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Y Kawamoto, M Ueda, H Ichikawa, A Miyama. 1979. Complement proteins and macrophages. 1. Quantitative estimation of factor B produced by mouse peritoneal macrophages.. https://doi.org/10.1111/j.1348-0421.1979.tb00529.x

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