Preparation of monospecific antiserums against porcine immunoglobulins, using agarose-linked immunosorbents.

E V De Buysscher; D T Berman

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PubMed · 51601

Preparation of monospecific antiserums against porcine immunoglobulins, using agarose-linked immunosorbents.

E V De Buysscher·D T Berman

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Abstract

Immunoglobulins IgG, IgA, and IgM were isolated from porcine serum and milk, and antiserums against the 3 immunoglobulin classes were prepared. Monospecificity of the antiserums for the gamma-, alpha-, and mu-chains was obtained by absorbing them in agarose-linked immunosorbent columns. These immunosorbents were prepared by linking IgG or IgA-IgM to CNBr-activated agarose. Contaminating anti-alpha2-macroglobulin antibodies in the anti-IgA and anti-IgM serums were removed with agarose-linked fetal globulins.

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Absorption Animals Cross Reactions Immune Sera Immunoelectrophoresis Immunoglobulin A Immunoglobulin G Immunoglobulin M Immunoglobulin alpha-Chains Immunoglobulin mu-Chains Immunoglobulins Methods Milk Sepharose Swine alpha-Macroglobulins Journal Article

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E V De Buysscher, D T Berman. 1975. Preparation of monospecific antiserums against porcine immunoglobulins, using agarose-linked immunosorbents.. https://pubmed.ncbi.nlm.nih.gov/51601/

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