PubMed · 5227787
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H S Crabb, K V Mortimer. 1967-04-18. Two-dimenisonal microdensitometry. A preliminary report.. https://pubmed.ncbi.nlm.nih.gov/5227787/
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Matrix metalloproteinase-3 (MMP-3 or stromelysin-1) hydrolyzes the Met(374)-Ser(375) (P3-P2), Glu(416)-Leu(417) and Ser(432)-Leu(433) peptide bonds in human alpha(2)-antiplasmin (alpha(2)-AP), the main physiological plasmin inhibitor. Cleavage is completely abolished in the presence of the MMP inhibitors EDTA or 1,10-phenanthroline. At enzyme/substrate ratio of 1:10 at 37 degrees C, alpha(2)-AP protein cleavage occurs with a half-life of 8 min, and is associated with rapid loss of inhibitory activity towards plasmin with a half-life of 5 min. alpha(2)-AP cleaved by MMP-3 does no longer form a stable complex with plasmin, as shown by SDS-PAGE, and does no longer interact with plasminogen, as shown by crossed immunoelectrophoresis with plasminogen added to the gel. These data are compatible with the removal of a COOH-terminal fragment containing the reactive site peptide bond and the plasmin(ogen)-binding site. In addition, MMP-3 cleaves the Pro(19)-Leu(20) peptide bond in alpha(2)-AP, thereby removing the fibrin-binding site from the inhibitor. A dysfunctional alpha(2)-AP variant (Ala-alpha(2)-AP or alpha(2)-AP Enschede), with an alanine insertion in the reactive site sequence converting it from a plasmin inhibitor into a substrate, was also efficiently cleaved by MMP-3 (half-life of 13 min at 37 degrees C and enzyme/substrate ratio of 1:10). Cleavage and inactivation of alpha(2)-AP by MMP-3 may constitute a mechanism favoring local plasmin-mediated proteolysis.
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A technique for determining the physical density distribution within an object using Compton scattered radiation is described. The technique is based on measuring the variation with the depth of 90 scattered radiation flux from an external monoenergetic photon source using a high-resolution solid-state detector. The measured scattered signal from an isolated volume element of the interrogated object is proportional to its electron density. For a definite region within the scanned object, the variation of the scattered yield with depth, along the direction of the incident beam, will give a counting curve with slope proportional to the density of the examined region. The spatial resolution of the system, determined by the collimator width, is 2 mm. The mathematical aspects of the technique are presented and the experimental data obtained in line scans of representative tissue equivalent materials are also shown to demonstrate the validity of the method.