PubMed · 5336129
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M Tsuchihashi. 1966. [Fetal adrenal cortex].. https://pubmed.ncbi.nlm.nih.gov/5336129/
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In order to search for the direct evidence of cellular response to low-dose radiation, we investigated wild-type p53 protein accumulation in several organs of mice after exposure to low doses of X-rays. Significant p53 accumulation within 24 h was observed in the mouse adrenal glands and pancreas after X-ray irradiation at 25 cGy and 50 cGy, but not at 100 cGy. In addition, differential p53 accumulation was also observed in the hypophysis, thymus, skin, lung, bone marrow and liver at different doses. In contrast, we observed no accumulation of p53 protein in the spleen, testis or kidney at any dose used in this experiment. The p53 accumulation induced by low-dose X-rays in mice was organ-specific. It is suggested that cell type and interactions with other signal transduction pathways of the hormone system, immune system and/or nervous system may contribute to the variable induction of p53 by low-dose X-ray irradiation. The association of organ-specific p53 response with radiosensitivity and cancer incidence by radiation in each organ was discussed.
Regulation of CYP17 gene expression in porcine Leydig cells was investigated in primary culture. We previously reported the sequence of the 5' upstream and much of the pig gene. (Zhang et al. (1992) Biochim. Biophys. Acta. 1131, 345-348). DNase I footprinting assays identified a region between -193 and -174 that was bound by nuclear proteins. Examination of the DNA sequence in this region revealed putative Sp1 and AP-2 binding sites, but gel retardation assays using an oligonucleotide from -198 to -168 as a probe revealed two specific DNA-protein complexes that were not Sp1 or AP-2. The oligonucleotide was cloned into a reporter gene containing a minimal porcine CYP17 promoter and the resultant construct was transiently transfected into porcine Leydig cells. This chimeric construct had both basal and cAMP-induced transcriptional activities. Southwestern blot identified a prominent binding of a nuclear protein around 68 kDa and a weaker binding of a nuclear protein around 110 kDa. Sequences between -250/+1 are highly homologous to those sequences from human, bovine and rodent CYP17 gene, but the -193/-174 region has no homology to those genes. Other regions of the porcine CYP17 were also important for the basal and cAMP-mediated regulation. Luciferase expression vectors were prepared with 5' flanking DNA from the porcine CYP17 gene and were expressed in primary culture of porcine Leydig cells. The region between -587/-325 was important for basal transcription, and a region of DNA between -325 and -140 was important for cAMP regulation.
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