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Letter: Lymphocyte transformation in vitro to paramyxovirus antigens in multiple sclerosis.

S Cunningham-Rundles·B Dupont·J B Posner·J A Hansen·R A Good
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Antigens, ViralCulture TechniquesHumansLymphocyte ActivationMeasles virusMultiple SclerosisMumps virusParamyxoviridaeRespirovirusComparative StudyJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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S Cunningham-Rundles, B Dupont, J B Posner, J A Hansen, R A Good. 1975-12-13. Letter: Lymphocyte transformation in vitro to paramyxovirus antigens in multiple sclerosis.. https://doi.org/10.1016/s0140-6736(75)92686-0

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Regions of the polyoma genome coding for T antigens.

The early region of the polyoma genome encodes three T antigens. We have analyzed the organization of the coding regions for the T antigens, using the nucleotide sequence of polyoma DNA and peptides derived from purified, radio-labeled T antigens, separated by two-dimensional electrophoresis and chromatography. We compared the peptides, predicted from the nucleotide sequence of the DNA, with those derived from the purified T antigens. We also compared chemically synthesized peptides, predicted from the DNA sequence, with observed peptides. The results show that the three polyoma T antigens are encoded in overlapping regions of the viral DNA, translated, in part, in two different reading frames.

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