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[Cardanolides from Anodendron paniculatum (Roxb.) A. DC].

J Polonia·H Jäger·J von Euw·T Reichstein
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CardanolidesGlycosidesSpectrum AnalysisJournal Article

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J Polonia, H Jäger, J von Euw, T Reichstein. 1970. [Cardanolides from Anodendron paniculatum (Roxb.) A. DC].. https://doi.org/10.1002/hlca.19700530602

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PubMed1976

Partition high-pressure liquid-chromatographic systems for the separation of digitalis glycosides of the cardenolide group.

Several multi-component liquid-systems have been investigated on silica gel SI-60 supports of particle size 10 mum. By using two solvent systems, it was possible to separate 14 digitalis glycosides, ranging from genins of relatively low polarity to the highly polar deacetyl-lanatosides. Solvents with good ultraviolet transparency at 220 nm (lambdamax. for the butenolide ring) were chosen in order to improve the sensitivity of detection. The technique should also permit the determination of by-products and degradation products of these drug substances. Detection limits are as low as 15 ng for a 5 mul injection, and separation times vary between 4 and 20 min. The reproducibility of the retention times and the baseline separations attainable make the systems suitable for quantitative work.

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