Antibody response after single dose human alloimmunization.

Abstract

Six individuals were immunized once, intradermally, with buffy coat cells from 100 ml of whole blood. The donor differed from the recipient for four HL-A antigens, two from the first and two from the second series. Two of the six recipients developed complement dependent cytotoxic antibodies, one of them against the immunizing donor only. Four of the six developed antibodies which were able to induce cell-mediated cytotoxicity, and which also inhibited the mixed lymphocyte culture interaction. Manily the responding cell in mixed lymphocyte culture was found to be inhibited. Antibodies with HL-A specificity seemed, at least partly, to be responsible for the observed effects.