PubMed · 55115
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A D Semenov. 1975. [Modification of staining histologic sections by van Gieson's method].. https://pubmed.ncbi.nlm.nih.gov/55115/
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The purpose of this study was to demonstrate the ability of a new method to discriminate between benign and malignant tissue. Tissue samples from three patients with bladder tumours and one patient with bladder diverticula have been examined by histophotometry. This method, based on the correlation of nuclear volume and nucleic acid contents per cell uses thick formalin fixed samples. The results are obtained by measurements averaging more than one hundred thousand cells per sample. By this histophotometric examination a clear differentiation between malignant and to differentiate between infiltrating and noninfiltrating tumour, as well as inflamed bladder tissue. The results obtained histophotometrically were compared with the histological findings.
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The substitution of lead nitrate for uranium nitrate as used in the Steiner silver impregnation method for demonstrating spirochetes in tissue sections is described. The use of lead nitrate provides a chemical substitute free of any potential radiation hazard without loss of staining specificity. The use of lead nitrate in place of uranium nitrate in the Dieterle method for staining Legionnaires' disease organisms is proposed.