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Uneven extraction of protein in Chinese hamster chromosomes during G-staining procedures.

S Matsukuma·T Utakoji
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AcetatesAzure StainsCell LineChromatinChromosomesDansyl CompoundsFluorescenceHydrochloric AcidNucleoproteinsStaining and LabelingTrypsinJournal Article

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S Matsukuma, T Utakoji. 1976. Uneven extraction of protein in Chinese hamster chromosomes during G-staining procedures.. https://doi.org/10.1016/0014-4827(76)90620-0

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Acetate is the preferred substrate for long-chain fatty acid synthesis in isolated spinach chloroplasts.

1. Commercially available [2-14C]pyruvate and [2-14C]malonate were found to contain 3-6% (w/w) of [14C]acetate. 2. The contaminating [14C]acetate was efficiently utilized for fatty acid synthesis by isolated chloroplasts, whereas the parent materials were poorer substrates. 3. Maximum incorporation rates of the different substrates examined were (ng-atoms of C/h per mg of chlorophyll): [1-14C]acetate, 2676; [2-14C]pyruvate, 810; H14CO3-, 355; [2-14C]malonate, 19. 4. Products of CO2 fixation were probably not a significant carbon source for fatty acid synthesis in the presence of exogenous acetate.

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