PubMed · 5592709
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E Rimbach, E Walker. 1967-04-22. [Liver function before and after gynecologic surgery].. https://pubmed.ncbi.nlm.nih.gov/5592709/
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BACKGROUND: Several thiocholine alkanoyl esters were newly synthesized and explored as substrates for the assay of human serum cholinesterase after being subjected to the Ellman reaction (Arch Biochem Biophys 1958;74:443-50 and Arch Biochem Biophys 1959;82:70-7). METHODS: We synthesized thiocholine ester iodides by the method of Renshow et al. (J Am Chem Soc 1938;60:1765-70). We examined solubility in H(2)O, substrate specificity serum for cholinesterase, (spontaneous) self-hydrolysis, storage stability, and reaction conditions for measurement of the activity of the enzyme. RESULTS: Isobutyryl and cyclohexane-carboxyl esters showed the best efficiency for the specific and stable assay of human serum cholinesterase. Aqueous solubility of each was >10 mmol/L, and the reactivity with acetylcholinesterase was negligible. For isobutyryl and cyclohexane-carboxyl esters, respectively, spontaneous hydrolysis in the aqueous phase was approximately 1/25 and approximately 1/175 slower than the enzymatic hydrolysis, and assays with these substrates were linear to 1800 and 3000 U/L, respectively. The K(m) values of these acylthiocholines with human cholinesterase were almost equivalent (6.9 x 10(-3) mmol/L). The substrates were stable in aqueous solution and in the solid state as the iodides for at least 5 years at 5 degrees C. CONCLUSIONS: The isobutyrate and cyclohexane-carboxylate of thiocholine are suitable for the specific assay of human serum cholinesterase.
The structures of biological life are formed in water. Their function depends on changes in the entropy of water. It is regulated by the cholinergic system. The initiating event is the ChE-splitting of water with liberation of free protons. They will draw electrons from the fairly inert dioxygen. The induced oxygen reactivity will give liberation and transfer of electrons and hydrodynamic pH-dependent changes in protein configurations. A multitude of sub-systems will be activated. The sequence of events normally ends with the formation of water, thus preventing uncontrolled radical chain reactions. Cholinergic receptors appear as restricting units of the general disordering entropy tendency. ChE-induced hydrodynamics is propagated to the inner of cells by the water soluble protons and the electrolytes. Especially Ca appear to have a strong influence on the hydrodynamic dipole moment of water. Because water is an integral structure of DNA genetics also will be influenced. Conditions caused by deprivation of oxygen or of reactive oxygen and disorders by hyperactivity and inactivity are briefly discussed. The CNS takes the shape of a large-scale quantum computer with a function far beyond our ability of immediate perception. The atomic nuclear proportions of quantum bits (qubits) will admit the functional one-cell unit of immune memory cells. Cholinergic hydrodynamics appear to substantiate the much discussed chaos theory.