Antibodies specific to two deoxyribotrinucleotide sequences.

S A Khan; T M Jacob

doi:10.1093/nar/4.9.3007

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Antibodies specific to two deoxyribotrinucleotide sequences.

S A Khan·T M Jacob

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Abstract

Antibodies to the deoxyribotrinucleotides dpApTpA and dpApApT were prepared by injecting the bovine serum albumin conjugates of the respective haptens in rabbits. The specificities of the antibodies were determined by estimating the inhibition of the binding of the tritiated haptens to the immunoglobulins by various nonradioactive mono- and oligonucleotides, using nitrocellulose membrane binding assay. Anti-dpApTpA and anti-dpApApT antisera were found to contain antibodies which were highly specific to the respective hapten sequence.

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Animals Antibodies Antibody Specificity Binding Sites, Antibody Kinetics Oligodeoxyribonucleotides Oligonucleotides Precipitin Tests Rabbits Serum Albumin, Bovine Structure-Activity Relationship Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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S A Khan, T M Jacob. 1977. Antibodies specific to two deoxyribotrinucleotide sequences.. https://doi.org/10.1093/nar%2F4.9.3007

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