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Clinical and immunological studies of timothy antigen D immunotherapy.

H S Nelson·G Brown·T P O'Barr·L B Branch·H Spaulding·J W Black
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Abstract

The response to preseasonal immunotherapy with aqueous grass extract, timothy antigen D, or water-soluble timothy (WST) in alginate was compared in patients sensitive to grass pollen. Injections of antigen D in alginate produced little evidence of clinical or immunologic response. Treatment with aqueous grass extract or WST in alginate, on the other hand, significantly reduced the seasonal rise in grass-specific IgE. Aqueous extract therapy was also associated with a decline in leukocyte sensitivity during the pollen season, while WST treatment produced the greatest rise in hemagglutinating antibodies.

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AlginatesAntigensHemagglutination TestsHistamine ReleaseHumansImmunoglobulin EImmunotherapyPlant ExtractsPoaceaeRadioimmunoassayRhinitis, Allergic, SeasonalSkin TestsClinical TrialComparative StudyControlled Clinical TrialJournal Article

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H S Nelson, G Brown, T P O'Barr, L B Branch, H Spaulding, J W Black. 1976. Clinical and immunological studies of timothy antigen D immunotherapy.. https://doi.org/10.1016/0091-6749(76)90062-2

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A study of the reaction catalysed by alginate lyase VI from the sea mollusc, Littorina sp.

The molecular weight of polymeric alginic acid digested by alginate lyase (poly(1,4-beta-D-mannuronide) lyase, EC 4.2.2.3) was determined at various stages of the lysis. Low molecular weigh fragments were detected only after 60-100% lysis. Some high molecular weight fragments remained intact even after addition of a fresh aliquot of enzyme to the digest. The enzyme showed maximal activity at pH 5.6 in 0.05 M salt. Enzyme activity was stimulated by addition of 7.5 mM CaCl2 and 0.2 M NaCl, when the pH optimum was between 8 and 8.5. Only mannuronic acid was detected at the reducing end of fragments after exhausive enzymolysis, reduction and hydrolysis. On studying the reaction products by NMR, a double-bound signal (sigma = 5.98 ppm) was observed. A considerable decrease in intensity of the D-mannuronic acid residue signal was detected after hydrolysis of alginate lyase VI on poly-(ManUA-GulUA), but not poly(GulUA). The results suggest that alginate lyase VI may be an endoalginate lyase that splits glycoside bonds only between two mannuronic acid residues.

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