15N nuclear magnetic resonance investigations on amino acids.

F Blomberg; W Maurer; H Rüterjans

doi:10.1073/pnas.73.5.1409

Home/Archive/PubMed

PubMed · 5722

15N nuclear magnetic resonance investigations on amino acids.

F Blomberg·W Maurer·H Rüterjans

View original source ↗Export metadata ↓

Abstract

15N nuclear magnetic resonance investigations of some amino acids were carried out in order to check the applicability of this method to biological problems. Because the natural abundance of the 15N isotope is not sufficient to get readable spectra in a reasonable time, 95% 15N isotope-enriched samples were used for the measurements. Besides the chemical shift values, the line widths, and the nuclear Overhauser enhancement factors, spin lattice relaxation times of the correspondent 15N resonances were measured as functions of pH had temperature.

Explore related subjects

Amino Acids Arginine Glutamine Glycine Hydrogen-Ion Concentration Kinetics Lysine Magnetic Resonance Spectroscopy Nitrogen Isotopes Proline Protons Temperature Water Journal Article

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

F Blomberg, W Maurer, H Rüterjans. 1976. 15N nuclear magnetic resonance investigations on amino acids.. https://doi.org/10.1073/pnas.73.5.1409

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2026

Chimeric structural isomer fragments as cost-efficient internal standards for amino acid quantification by mass spectrometry.

Amino acid (AA) profiles from body fluids such as blood and urine are clinical indicators for diagnosing metabolic and hepatic diseases. Current quantitative methods, such as liquid chromatography-mass spectrometry (LC-MS) with isotopically labelled internal standards (ISs), are costly and technically demanding. This study proposes a cost-efficient alternative using structural isomers as ISs in a direct liquid infusion (DLI) tandem mass spectrometry (MS/MS) approach. The method leverages chimeric spectra and fragment intensity ratios to quantify AAs, demonstrating high linearity and precision even with a 3D ion trap mass analyser. This approach offers a viable strategy for AA quantification in preventive medicine, particularly for screening metabolic diseases such as phenylketonuria, diabetes, and liver dysfunction.

Amino Acids↗

PubMed1979

[Methods of screening for cystinuria. Proposed use of thin-layer chromatography].

Explore the source record for details and available documents.

Amino Acids↗

PubMed1979

Identification and quantitation of nucleosides, bases and other UV-absorbing compounds in serum, using reversed-phase high-performance liquid chromatography. I. Chromatographic methodology.

A comprehensive investigation of the high-performance liquid chromatographic separation of nucleosides, their bases and other low-molecular-weight UV-absorbing compounds that might be found in serum is reported. A buffer-methanol gradient was used in conjunction with chemically bonded, microparticulate columns to separate many of the biologically important compounds under study in minimal time with maximal resolution. Retention data, absorbance ratios (280/254 nm) and fluorescence responses are reported for 86 nucleosides, bases, nucleotides and other UV-absorbing compounds commonly encountered in biological studies.

Amino Acids↗