PubMed · 5763949
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1969. Current techniques for stapes surgery.. https://pubmed.ncbi.nlm.nih.gov/5763949/
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The use of an active Nafion substrate is shown to enhance the performance of MALDI MS. The use of a Nafion substrate with certain matrices can significantly enhance the signals obtained over those observed with a stainless steel probe substrate. Analytes can often be observed with the use of the Nafion substrate that cannot be easily observed with the standard MALDI procedure, and usually a much wider range of peaks can be observed using MALDI from the Nafion substrate than with any single matrix on a stainless steel substrate. This enhancement of signal from the Nafion substrate is observed only with the sequential deposition of the sample and the matrix onto the Nafion film. If the analyte and matrix are premixed, then the effect is not observed. The use of the Nafion substrate has been shown to be particularly effective in analyzing real biological mixtures without prepurification. This has been demonstrated for various samples including the analysis of the products of chemical digests of proteins, protein profiling in milk and egg white samples, cell lysate analysis, and oligonucleotide detection.
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Due to the loss of topesthesia and to the impairment of the vasomotorium in the paralyzed area, patients suffering from tetraplegia have a high risk from pressure sores. Bed sores respond successfully to therapy if, on observing the relevant basic nursing procedure--requiring in particular that the damaged areas of skin be consistently relieved from pressure by means of special positioning of the patient--the sores are subjected to intensive cleansing via surgical and/or enzymatic débridement, and are conditioned with the synthetic skin substitute Epigard. This is achieved as a result of therapeutic procedure followed in a series of methodical steps. Specific treatment with antibiotics before conditioning the wounds supplements these measures and facilitates the spontaneous healing of the lesions or, in the case of larger bed sores, the optimal preparation of the wound bed prior to skin grafting and plastic surgery.