PubMed · 5932328
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1966. Quantitative methods for investigating the microcirculation.. https://pubmed.ncbi.nlm.nih.gov/5932328/
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We describe a simple new method for computing two-dimensional velocity vector fields of blood flows in microvascular networks. This method, known as optical flow, requires a time sequence of two or more images obtained by a process such as conventional videomicroscopy. There is no need to distinguish individual cells provided clear variations in the light intensity levels are present in the images. The result of the computation reveals the velocity distribution in the microvascular network and the geometry of the blood vessels. Although various implementations of the optical flow technique exist, we have found that the spatial correlation algorithm of Anandan performs best for microcirculatory flows. This report is an introduction to the application of optical flow to the microcirculation and provides instructions on how to obtain and run the codes.
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The red blood cell deformability was determined in 20 children suffering from cystic fibrosis (CF), and the results were compared with the data on 20 healthy children. In CF, a pronounced impairment of the deformability was found.