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Urea-induced structural changes in chromatin obtained by sedimentation.

R E Harrington
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Abstract

Sedimentation coefficients have been determined for fractionated preparations of whole and stripped (depleted of very lysine-rich histones and non-histone proteins) chicken erythrocyte chromatin fragments in 0-10 M urea. Significant differences in urea effects are observed between these preparations; differences which can be interpreted structurally by use of Kirkwood's dynamical theory of the translational frictional coefficient. This type of analysis implies that urea-induced chain-swelling in stripped chromatin is due largely to the urea effect upon the constituent nu-bodies, whereas the much larger swelling observed in whole chromatin appears to involve also the effect of urea upon the region between adjacent nu-bodies.

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AnimalsCentrifugation, Density GradientChickensChromatinErythrocytesNucleic Acid ConformationProtein ConformationUreaJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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R E Harrington. 1977. Urea-induced structural changes in chromatin obtained by sedimentation.. https://doi.org/10.1093/nar%2F4.11.3821

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