PubMed · 5956314
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H L Kay. 1966-03-19. Apparatus for dynamic thin-layer chromatography.. https://doi.org/10.1038/2091237a0
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The interaction between 15 steroidal drugs and carboxymethyl-gamma-cyclodextrin (CM-gamma-CD) was studied by reversed-phase charge-transfer thin-layer chromatography and the relative strength of interaction was calculated. CM-gamma-CD formed inclusion complexes with each compound, the complex always being less hydrophobic than the uncomplexed drug. The inclusion-forming capacity of drugs differed considerably depending on their chemical structures. The linear correlation between the hydrophobicity and specific hydrophobic surface area of anticancer drugs indicated that they can be considered as a homologous series of compounds, although their chemical structures are different. Hydrophobicity of drugs significantly influenced the strength of interaction, indicating the involvement of hydrophobic forces in the binding of drugs to CM-gamma-CD. The marked influence of CM-gamma-CD on the hydrophobicity of drugs suggests that this interaction may modify the biological properties (adsorption, uptake, half-life, etc.) of drug-CM-gamma-CD complexes drug, resulting in modified efficacy.
Several methods for the quantitative analysis of sialic acids in glycoconjugates require that the sialic acid glycosidic bond be cleaved prior to assay. The conventional (L. Svennerholm, (1958) Acta Chem. Scand. 12, 547-554) procedure for the hydrolysis of complex carbohydrates such as gangliosides employs 0.1 n H+ at 80 degrees C for 60 min. Under these conditions, we find that the monosialoganglioside (GM1) yields less than 50% of the total sialic acid. However, 90% recovery of sialic acid was achieved by supplementing the hydrolysis mixture with 0.2% sodium dodecylsulfate (SDS) and increasing the temperature to 85 degrees C. During hydrolysis under these conditions, N-acetylneuraminic acid (NAN) is degraded at about 7% per hour. If the analytical values for GM1 are corrected for degradation, the recovery of NAN is essentially quantitative.
The 5-(n)-alkylresorcinol fraction of the epicuticular waxes of Hordeum vulgare seeds appeared to be responsible for their in-born resistance to pathogenic fungi such as Aspergillus niger and Penicillium crysogenum. The antifungal properties of this fraction were evaluated qualitatively and quantitatively with a novel bioassay where the extreme lipophilicity of these compounds was taken into account. The minimum inhibitory concentration in the fungi tested ranged from 5.6 to 10 micrograms cm-2 for the alkyresorcinols. The behaviour of the different cultivars against these fungi could be predicted by measuring the natural amount of resorcinols of each variety by TLC-scanning densitometry. The ranking of cultivars thus established correlated well with the field behaviour of each cultivar, providing a useful and rapid method for predicting the behaviour against fungi of new varieties being developed.