PubMed · 5971895
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E Afonso. 1966. Quantitative immunoelectrophoresis. A simplified method.. https://doi.org/10.1016/0009-8981(66)90051-9
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OBJECTIVE: To establish a specific method for determination of IgG in IVIG. METHODS: The IVIG samples were disposed by acylation and analyzed by Rocket Immunoelectrophoresis. The amount of the IgG in IVIG was calculated by an IgG standard curve. RESULTS: The standard curve by this method has a very good linear relationship: r = 0.9967. The reproducibilities with 3 bathes of IVIG samples (n = 6) are 5.37% +/- 0.21%, 5.28% +/- 0.39% and 5.80% +/- 0.30%. CONCLUSION: This method could be adapted to regular assay in blood products.
The mass ratio of nitrous oxide reductase to total protein in the soluble protein fraction of Pseudomonas aeruginosa P2 was highest in cells grown on nitrate, decreased in cells grown on N(2)O following the exhaustion of the initial charge of nitrate, and was nearly zero in cells exposed solely to N(2)O.
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