PubMed · 5980316
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N Tanaka. 1966. [New liver function test using indocyanine green].. https://pubmed.ncbi.nlm.nih.gov/5980316/
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This paper reviews groundwater pollution caused by the disposal of untreated effluents of a dye factory located 20 km to the west of Nicosia (Cyprus). The task of the work was to investigate the nature of the pollution and differentiate it from other possible pollution sources in the area. It focused on toxicity testing and biofractionation in order to address the most toxic pollutants and, on the evaluation of GC/FID profiles for investigating the connection between groundwater pollution and the effluents. This connection was successful due to a multiple comparison amongst the GC/FID profiles resulting from the polluted groundwater, the water from the reference areas and the dye effluents.
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A direct dye-binding procedure was established for the quantification of protein after its immobilization on a solid phase, using IgG and BSA as model proteins. The assay, which in the range 0-5 mg protein/ml gel correlates well with indirect protein determination by A280 as well as determination of protein hydrolyzed from the gel, is based on a modified Bradford dye-binding assay. As the protein coupled to the gel binds the dye, a decrease in A465 of the supernatant is measured. Three solid supports commonly used for protein immobilization (Sepharose, Sephadex, Sephacryl) were found to be compatible with the dye-binding assay while nonspecific dye binding was found to HEMA gels. Protein was coupled to Sephacryl S-1000 using three different activation methods (aldehyde, hydrazine, and adipic acid dihydrazide). Artifactual dye-binding was not observed using any of the three different "linkers." The assay is easily carried out and represents a useful tool, e.g., when optimizing procedures for protein immobilization.