PubMed · 5981436
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J Mercadal-Peyrí, M Bassas-Grau, C de Martín-Gassó, J O Mercadal-Peyrí. [Fibrosarcoma].. https://pubmed.ncbi.nlm.nih.gov/5981436/
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A method for combined light and fluorescent microscopic imaging of nucleolar organizer regions and cellular rRNA is described. Nucleolar organizer regions were detected by silver staining (Ag-NOR), and rRNA was detected by fluorescent in situ hybridization (FISH). MG-63 human fibrosarcoma cells were silver stained prior to in situ hybridization. To quantitate Ag-NOR within individual cells, brightfield images were digitized, and the total Ag-NOR area/nucleus was determined. Fluorescent images were digitized, and the total cellular fluorescence was calculated after correction for nonuniformity of illumination. By using this method, it was shown that the Ag-NOR procedure did not significantly affect the fluorescence intensity related to FISH. Furthermore, the hybridization procedure did not interfere with quantitation of Ag-NOR. With this method, both Ag-NOR and rRNA product can be quantitated within the same cell. Because the relationship of rRNA content to cell proliferation is well established, correlation to quantitative Ag-NOR parameters within individual cells will contribute to the better definition of the relationship of quantitative Ag-NOR indices with cellular proliferation.
Sarcomas are rare and malignant tumors of mesenchymatous origin. Numerous histological sub-types have been described. 10 to 15% of all sarcomas are located to head and neck. The more frequent histological types in this localization are fibrosarcoma, malignant fibrous histiocytoma and rhabdomyosarcoma. In this paper a general review of histological classification of sarcomas is proposed with a particular emphasis on head and neck sarcomas.
This article provides an overview of the classification of soft-tissue sarcomas as influenced by modern histopathologic techniques. The overview is followed by a practical grading system for these tumors based on a study of 282 eligible patients. The primary tumors of this trial were adequately treated. The quantitative data (mitotic count and size of the tumor) were based on the results of the statistical analysis.