PubMed · 5995545
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
N Leon. 1966. [Human cytogenetics].. https://pubmed.ncbi.nlm.nih.gov/5995545/
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
During the last few years molecular biology methods expanded into human cytogenetics. This is in close connection with advanced technologies of DNA probes preparation and possibilities of their non-radioactive labelling by means of enzymatic incorporation of modified nucleotides as well as their hybridization with complementary DNA of chromosomes and interphase nuclei. FISH became a useful method in the clinical research. We present the short review of FISH methodologies and their applications for studies of translocation, deletions, amplifications and other chromosomal rearrangements in genetic and oncologic patients. The sensitivity of these methods is approximately 1-10 kb and therefore precise localization of genes on chromosomes is possible. Except gene mapping FISH can be used for comparative genomic mapping (CGH) and for identification of chromosomal changes of tumor cells.
Explore the source record for details and available documents.
In this review we summarize the most recent advances of flow and image cytometry, cytogenetics and molecular genetics of tumours and tumour-like lesions of the thyroid, especially focusing on the putative relationship between nodular goitres and adenomas, the differential diagnosis of benign and malignant neoplasms and the pathogenesis and prognosis of carcinomas. Data obtained by in situ hybridization and immunohistochemical detection of proliferative markers, metal binding proteins and oncogenes, anti-oncogenes, growth factors and growth factor receptors are also used whenever appropriate.