Changes in chromosome complement in long-term pea callus cultures.

H Kallak; L Järvekülg

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PubMed · 605710

Changes in chromosome complement in long-term pea callus cultures.

H Kallak·L Järvekülg

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Abstract

A prolonged callus culture from pea (Pisum sativum L. var. Kiir) cotyledons subcultured for 7.5 years on Torrey's solid medium was examined cytologically. The initially (and up to 3.5-year cultivation) predominantly diploid pea callus strain changed into triploid. The frequency of aberrant ana- and telophases increased during 5 year cultivation from 9 to 40 per cent and thereafter returned to the initial rate maintaining it at least for one year of subculturing. Some possible mechanisms of chromosomal variation tendencies in vitro are discussed.

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H Kallak, L Järvekülg. 1977. Changes in chromosome complement in long-term pea callus cultures.. https://pubmed.ncbi.nlm.nih.gov/605710/

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