PubMed · 6072339
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W G Walter. 1967-07-10. Dieffenbachia toxicity.. https://pubmed.ncbi.nlm.nih.gov/6072339/
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Kochia scoparia was collected periodically during the growing seasons of 1984, 1985 and 1986. The 10, 16 and 16 samples, respectively, from these years were air dried, hammer milled and analyzed at a later date for soluble and total oxalates. An additional 6 samples from New Mexico were analyzed from the 1987 growing season. A maximum of 4.7% soluble oxalates and 11.4% total oxalates were present in 1986 Colorado kochia following heavy rainfall. This concentration of soluble oxalate could cause chronic oxalate poisoning (alkalosis) in cattle.
A simple method is described for the measurement of urinary oxalate. Oxalate decarboxylase is coupled with NAD+ requiring formate dehydrogenase and the result recorded spectrophotometrically. Accurate determination can be carried out either on urine or a citrate extract or urine. Using the citrate extract procedure, the urinary samples can be stored for at least 3 months without any effect on the oxalate content.
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