PubMed · 6080071
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B Chapman. 1967-12-30. Polystyrene replicas for scanning reflexion electron microscopy.. https://doi.org/10.1038/2161347a0
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Some basic quantitative analysis techniques of digitized high resolution atomic image are developed in this paper. We describe how to divide the atomic image into small independent areas with special structure information by the valley mesh segmentation method. The procedure is with regard to the bright contrast spot segmentation of atomic images. We suggest several ways for bright contrast spot localization in images. Calculation routines for peak detection and weighted local position average methods for this purpose are given.
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Trimmatostroma abietis initially grew as hyphae when grown in various media containing yeast extract or bactopeptone. It grew as segmented elements (lumbricoid elements) characterized by bidirectional growth, when grown in Czapek-Dox broth or yeast nitrogen base supplemented with 1% glucose. A lumbricoid element usually was 10-70 microns in length, with transverse septation only and contained 3 to 15 cells. Growth and propagation, as revealed by time-lapse photomicrography occurred as follows. Elements usually grew by apical elongation without widening; after simple apical elongation adjacent parts of two central cells eventually started to grow, resulting in the separation of the element into two.