A decrease of RNA synthesis during macrophage activation with lipopolysaccharide--an association with novel regulating factors.

Y Okai

doi:10.1016/0165-2478(84)90006-3

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A decrease of RNA synthesis during macrophage activation with lipopolysaccharide--an association with novel regulating factors.

Y Okai

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Abstract

RNA synthesis during macrophage activation with lipopolysaccharide (LPS) was analyzed in mice. The RNA synthesis of macrophages was considerably increased early after LPS stimulation, but drastically decreased at a later period. The decrease of RNA synthesis was observed in mouse strains responding to LPS such as BALB/c, ATL and ddY, but not in nonresponder strain, C3H/HeJ. Furthermore, the decrease of RNA synthesis occurred in both alpha-amanitin resistant and sensitive RNA polymerase reactions. However, RNA polymerases in nuclei were not changed significantly in LPS-treated macrophages, but the factors affecting RNA polymerase reactions were drastically changed. Amongst these factors, the stimulating factors were decreased and a novel repressing factor was remarkably increased. The factors were partially characterized and their significance in macrophage activation is discussed.

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Animals DNA-Directed RNA Polymerases In Vitro Techniques Lipopolysaccharides Macrophage Activation Macrophages Mice Mice, Inbred Strains RNA Comparative Study Journal Article

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Y Okai. 1984. A decrease of RNA synthesis during macrophage activation with lipopolysaccharide--an association with novel regulating factors.. https://doi.org/10.1016/0165-2478(84)90006-3

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