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PubMed · 6088071

Human transforming growth factor-alpha: precursor structure and expression in E. coli.

R Derynck·A B Roberts·M E Winkler·E Y Chen·D V Goeddel
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Abstract

Transforming growth factor-alpha (TGF-alpha) is secreted by many human tumors and can induce the reversible transformation of nontransformed cell lines. Using long synthetic deoxyoligonucleotides as hybridization probes we isolated an exon coding for a portion of TGF-alpha from a human genomic DNA library. Utilizing this exon as a probe, a cell line derived from a human renal cell carcinoma was identified as a source of TGF-alpha mRNA. A cloned TGF-alpha cDNA was isolated from a cDNA library prepared using RNA from this cell line, and was found to encode a precursor polypeptide of 160 amino acids. The 50 amino acid mature TGF-alpha produced by expression of the appropriate coding sequence in E. coli binds to the epidermal growth factor receptor and induces the anchorage independence of normal mammalian cells in culture.

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AdenocarcinomaAmino Acid SequenceBase SequenceCell LineCell Transformation, NeoplasticCloning, MolecularDNAEpidermal Growth FactorErbB ReceptorsEscherichia coliGenesHumansKidney NeoplasmsNeoplasm ProteinsNucleic Acid HybridizationPeptidesPlasmidsRNA, MessengerReceptors, Cell SurfaceTransforming Growth FactorsJournal Article

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R Derynck, A B Roberts, M E Winkler, E Y Chen, D V Goeddel. 1984. Human transforming growth factor-alpha: precursor structure and expression in E. coli.. https://doi.org/10.1016/0092-8674(84)90550-6

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