Echinomycin binding sites on DNA.

M M Van Dyke; P B Dervan

doi:10.1126/science.6089341

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PubMed · 6089341

Echinomycin binding sites on DNA.

M M Van Dyke·P B Dervan

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Abstract

The preferred binding sites of echinomycin on DNA can be determined by a method called "footprinting." A 32P end-labeled restriction fragment from pBR322 DNA is protected by binding to echinomycin, and cleaved by a synthetic DNA cleaving reagent, methidiumpropyl--EDTA . Fe(II); the DNA cleavage products are then subjected to high-resolution gel analyses. This method reveals that echinomycin has a binding site size of four base pairs. The strong binding sites for echinomycin contain the central two-base-pair sequence 5'-CG-3'. From an analysis of 15 echinomycin sites on 210 base pairs of DNA, key recognition elements for echinomycin are contained in the sequences (5'-3') ACGT and TCGT (A, adenine; C, cytosine; G, guanine; T, thymine).

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Base Sequence Binding Sites DNA DNA Restriction Enzymes Echinomycin Electrophoresis Escherichia coli Plasmids Quinoxalines Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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M M Van Dyke, P B Dervan. 1984-09-14. Echinomycin binding sites on DNA.. https://doi.org/10.1126/science.6089341

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