A Spiroplasma tRNA gene cluster.

M J Rogers; A A Steinmetz; R T Walker

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PubMed · 6096303

A Spiroplasma tRNA gene cluster.

M J Rogers·A A Steinmetz·R T Walker

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Abstract

Using molecular cloning techniques, a clone containing a 7-kb insert of Spiroplasma species BC3 DNA that hybridized strongly to total labeled Spiroplasma tRNA was identified. Sequence analysis of a portion of this recombinant plasmid identified a cluster of tRNA genes. The gene order was as follows: tRNACys-tRNAArg-tRNAPro-tRNAAla-tRNAMet-tRNAIle and a portion of tRNASer All the genes encode the 3'-terminal CCA and have very high A + T and relatively long intergenic regions. An RNA polymerase promoter site was found upstream of the tRNACys gene. The tRNA gene cluster found in Spiroplasma can be compared with a similar Bacillus subtilis gene cluster, which raises interesting questions concerning gene organization and transcription.

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Base Sequence DNA Restriction Enzymes DNA, Bacterial Genes, Bacterial Genetic Linkage Hydrogen Bonding RNA, Transfer Spiroplasma Comparative Study Journal Article Research Support, Non-U.S. Gov't

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M J Rogers, A A Steinmetz, R T Walker. 1984. A Spiroplasma tRNA gene cluster.. https://pubmed.ncbi.nlm.nih.gov/6096303/

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