Chromatin structure and DNA replication.

W Pülm; R Knippers

doi:10.1007/978-1-4684-8730-5_13

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Chromatin structure and DNA replication.

W Pülm·R Knippers

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Abstract

The structure of (3H) thymidine pulse labeled, replicating chromatin differs from that of non replicating chromatin by several operational criteria which are related to the higher nuclease sensitivity of replicating chromatin. We summarize the structural changes that we observe using replicating chromatin as substrate for micrococcal nuclease. The data suggest a more extended configuration of replicating compared to non replicating chromatin. We use these data to discuss a model of the chromatin structure in the vicinity of replication forks. Finally, we present data to show that the reversion of structural changes in replicative chromatin depends on continued DNA replication.

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Animals Cell Nucleus Chromatin DNA Polymerase II DNA Replication Histones In Vitro Techniques Micrococcal Nuclease Simian virus 40 Viral Proteins Comparative Study Journal Article Research Support, Non-U.S. Gov't

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W Pülm, R Knippers. 1984. Chromatin structure and DNA replication.. https://doi.org/10.1007/978-1-4684-8730-5_13

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