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Ram sperm mitochondrial DNA.

J Fisher·J Langsam·B Bartoov
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Abstract

Circular DNA was isolated from mitochondrial fractions of ram spermatozoa by SDS treatment followed by convex sucrose gradient centrifugation. The DNA had a contour length of 5.0 micron. Its buoyant density was 1.6983 g cm-3, which was smaller than two nuclear DNA components with buoyant densities of 1.6999 and 1.7156 g cm-3, found in ram spermatozoa. The Tm of the mitochondrial DNA was 69.7 degrees C. The mole fraction G+C calculated from the buoyant density and melting temperature was 39.1% and 38.6%, respectively.

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AnimalsCentrifugation, Density GradientDNA, CircularDNA, MitochondrialMaleNucleic Acid ConformationNucleic Acid DenaturationSheepSpermatozoaJournal Article

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J Fisher, J Langsam, B Bartoov. 1977. Ram sperm mitochondrial DNA.. https://doi.org/10.1016/0309-1651(77)90091-1

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