PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 6126537

A stability-indicating assay for cefotaxime utilizing thin-layer chromatography with fluorescence detection.

H Fabre·N Hussam-Eddine
View original source ↗Export metadata ↓

Abstract

A t.l.c. method on silicagel plate with a fluorescence indicator has been carried out to increase the native fluorescence of cefotaxime and four of its degradation products. A simultaneous improved fluorimetric quantitation of the compounds in situ is proposed using a chromatogram spectrophotometer. The detectability (0.20 microgram) shows that the procedure is twenty times more sensitive than high-pressure liquid chromatography with u.v. detection; it allows the determination of traces level (1%) degradation products.

Explore related subjects

CefotaximeChromatography, Thin LayerDrug StabilitySpectrometry, FluorescenceJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

H Fabre, N Hussam-Eddine. 1982. A stability-indicating assay for cefotaxime utilizing thin-layer chromatography with fluorescence detection.. https://doi.org/10.1111/j.2042-7158.1982.tb04749.x

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2006

Serotype distribution and antimicrobial susceptibility of S. pneumoniae causing invasive disease in Thai children younger than 5 years old, 2000-2005.

In order to predict the potential benefit of pneumococcal conjugate vaccines (PCV), we evaluated the serotype coverage of the 7-, 9-, 11- and 13-valent PCV over the isolates causing invasive pneumococcal disease (IPD) in Thai children. One hundred and fifteen Streptococcus pneumoniae isolates from sterile sites in children younger than 5 years old between 2000 and 2005 were serotyped. The coverages of 7-, 9-, 11-, and 13-valent PCV were 69%, 73.8%, 73.8% and 85.7% in children younger than 2 years, and 73.9%, 77.4%, 77.4% and 87.8% in children younger than 5 years of age, respectively. 69.6% and 22.6% of the isolates were non-susceptible to penicillin and cefotaxime. 7-valent PCV covered 89% and 100% of penicillin and cefotaxime non-susceptible isolates.

Cefotaxime↗
PubMed2006

A nosocomial outbreak of Escherichia coli producing CTX-M-15 and OXA-30 beta-lactamase.

During a survey of the prevalent subtypes of extended-spectrum beta -lactamases in a university hospital in Korea, a nosocomial outbreak of Escherichia coli producing CTX-M-15 and OXA-30 beta -lactamases was detected. The outbreak comprised various infections, including bloodstream infections and colonization, and persisted for several months in various areas of the hospital.

Cefotaxime↗