A simple immunofluorescence assay for C1q binding.

E Linder

doi:10.1016/0022-1759(80)90189-1

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A simple immunofluorescence assay for C1q binding.

E Linder

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Abstract

Antibody-independent binding of B1q to Crithidia luciliae kinetoplast DNA was demonstrated by immunofluorescence microscopy. The method allows detection of C1q in human sera diluted 1/20--1/40 and in 5--10 micrograms/ml concentrations of isolated C1q. Various substances known to interfere with the early events of complement activation inhibited binding of C1q to kinetoplasts. The method may be used as a functional assay for serum C1q.

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Binding Sites, Antibody Binding, Competitive Complement C1 Complement Pathway, Classical Crithidia DNA Fluorescent Antibody Technique Humans Receptors, Complement gamma-Globulins Journal Article

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E Linder. 1980. A simple immunofluorescence assay for C1q binding.. https://doi.org/10.1016/0022-1759(80)90189-1

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