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Poly(U)-agarose affinity chromatography: specific, sensitivity selectivity, and affinity of binding.

L A Phillips·R H Pang·J J Park·V W Hollis·F Famuyiwa
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Abstract

These studies were done to determine four basic intrinsic properties of poly(U)-agarose affinity columns. Specificity of binding studies demonstrated that binding to these columns is highly specific with greater than 90% complementary binding and less than or equal to 3% noncomplementary binding. Sensitivity of binding studies indicated that a minimum sequence of 10 adenylates is required for detectable complementary binding. Selectivity of binding studies revealed that nonsequential adenylates in native RNAs and randomly distributed adenylates in synthetic poly(A)-poly(C) co-polymers did not bind to poly(U)-agarose affinity columns. Whereas, affinity of binding studies demonstrated that A=U complementary base pairing is independent of chain-lengths of greater than or equal to 25 adenylates and dependent of chain-lengths of less than 25 adenylates. Thus the data demonstrates that poly(U)-agarose affinity chromatography is scientifically sound and expedient for the detection and isolation of poly(A)-containing cellular and viral RNAs.

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Chromatography, AffinityEscherichia coliOligoribonucleotidesPoly APoly UPolyribonucleotidesRNA, BacterialRNA, TransferSepharoseJournal Article

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L A Phillips, R H Pang, J J Park, V W Hollis, F Famuyiwa. 1980. Poly(U)-agarose affinity chromatography: specific, sensitivity selectivity, and affinity of binding.. https://doi.org/10.1080/00327488008061715

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